Comparative study of the performance of two different luciferases for the analysis of fumonisin B1 in wheat samples

The development of two different immunoassays for the determination fumonisin B1 in wheat samples is reported. A previously described mimopeptide (FB1) was used to produce fusion proteins combination with luciferases: Gaussia luciferase (GLuc) and NanoLuc (NLuc). production, expression based on these (A2-GLuc A2-NLuc) detailed. assay showing best performance, A2-NLuc, a limit detection 0.61 ng mL−1 dynamic range from 1.9 95 mL−1, employed analysis spiked samples, reference matrix material, as well naturally contaminated samples. recoveries obtained were acceptable, between 81.5 109 %, relative standard deviations lower than 14 %. validated by liquid chromatography coupled tandem mass method.